目的：研究人参皂甙Rg 1对原代培养胎鼠脑神经细胞存活和可塑性的影响。方法：实验分为：实验组（人参皂甙Rg 1 1 mg/L,10 mg/L,100 mg/L），阳性药物对照组（bFGF 20 μg/L）以及空白对照组。相差倒置显微镜观察细胞生长情况，并测量细胞突起的长度；用MTT法测定培养细胞的存活率；Western-blot法检测神经生长相关蛋白GAP-43和神经丝蛋白NF-200的表达。结果：（1）细胞平均突起长度：实验高中剂量组神经元突起的平均长度均长于对照组。（2）MTT值：实验高中低剂量组的灰度均明显大于对照组。（3）GAP43和NF 200的表达：实验高中剂量组的蛋白表达均明显大于对照组。结论：人参皂甙Rg 1对于体外培养的胎鼠脑神经细胞的存活有较强的维持作用，并能促进突起生长，使神经可塑性相关蛋白表达上调。
Citation：
PENG Tao,Yinghui,CHEN Shaohong,et al.. Effect of Ginsenoside Rg1 on the Survival Rate andNeuronal Plasticity of Primary Cultured Cortical Neurons. West China Medical Journal, 2008, 23(1): 106-107. doi:
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Copyright © the editorial department of West China Medical Journal of West China Medical Publisher. All rights reserved
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- 2. 李会娟,樊均明.人参皂甙Rgl的药理作用及机制[J].四川医学,2006,27:1007-1008..
- 3. 刘颖,李玺,袁海峰.人参皂甙Rg1促进学习记忆作用研究进展[J].中国中西医结合杂志,2006,26(10):956-960..
- 4. D.L.斯佩克特,R.D.戈德曼,L.A.莱因万德.细胞实验指南[M].北京:科学出版社,2001:62-65..
- 5. 郭琴,毛萌,罗小丽,等.神经元原代培养方法的改进[J].华西医学,2007,22(3):566-568..
- 6. Tao Peng,Yinghui Liu,Chunlei Yang,et al.A New Peptide with Membrane-permeable Function Derived from Human Circadian Proteins[J].Acta Biochim Biophys Sin,2004,36(9):629-636..
- 7. 温进坤,韩梅.医学分子生物学理论与研究技术[M].北京:科学出版社,2002:225-232..
- 8. Skene JHP.Axonal-growth associated proteins[J].Annu Rev Neurosci,1989，12:127-156..
- 9. Julien JP,Mushynski WE.Neurofilaments in health and disease[J].Prog Nucleic Acid Res Mol Biol,1998,61:1-23..
- 10. Cheng B,Mattson MP.NGF and bFGF protect rat hippocampal and human cortical neurons against hypoglycemic damage by stabilizing calcium homeostasis[J].Neuron,1991,7:1031-1041..
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