目的:采用常压间歇性低氧(intermittent hypoxia,IH)大鼠的动物模型,观察间歇性常压低氧预处理的促血管生成作用。方法:成年雄性Wistar大鼠25只,体重210~215 g,随机分为2大组:对照组(C组,n=5)和间歇性低氧预处理组(IH组,n=20)。IH组动物进行间歇性低氧预处理(4 h/d,间歇缺氧1 d者为IH1组,7 d者为IH2组,14 d者为IH3组,28 d者为IH4组),按计划完成实验后测定心肌血管内皮生长因子(VEGF) 蛋白表达及毛细血管密度。结果:间歇性低氧预处理大鼠心肌VEGF蛋白表达增加,心肌毛细血管密度增高。结论:间歇性低氧预处理能促进大鼠心肌内的血管生成,其机制可能与心肌VEGF表达增高有关。
Citation:
ZHANG Wenyong,WANG hang,LI Qiu,et al.. The Angiogenesis Effect of Intermittent Hypoxia Precondition on Myocardium in Rats. West China Medical Journal, 2008, 23(1): 108-109. doi:
Copy
Copyright © the editorial department of West China Medical Journal of West China Medical Publisher. All rights reserved
1. |
Murry CE,Jennings RB,Reimer KA.Preconditioning with ischemia:adelay of lethal cell injury in ischemic myocardium[J].Circulation,1986,74(5):1124-1136..
|
2. |
Fagan KA.Selected Contribution:Pulmonary hypertension in mice following intermittent hypoxia[J].J Appl Physiol,2001,90(6):2502-2507..
|
3. |
Koyama T,Xie Z,Gao M,et al.Adaptive changes in the capillary network in the left ventricle of rat heart[J].Jpn J Physiol,1998,48(4):229-41.
|
4. |
Neufeld G,Cohen T,Gengrinovitch S,et al.Vascular endothelial growth factor (VEGF) and its receptors[J].FASEB J,1999,13(1):9-22..
|
5. |
Liu LX,Lu H,Luo YX,et al.Stabilization of vascular endothelial growth factor mRNA by hypoxia-inducible factor 1[J].Biochem Biophys Res Commun,2002,291(4):908-914..
|
6. |
Banai S,Shweiki D,Pinson A,et al.Upregulation of vascular endothelial growth factor expression induced by myocardial ischaemia:implications for coronary angiogenes[J].Cardiovasc Res,1994,28(8):1176-1179.
|
- 1. Murry CE,Jennings RB,Reimer KA.Preconditioning with ischemia:adelay of lethal cell injury in ischemic myocardium[J].Circulation,1986,74(5):1124-1136..
- 2. Fagan KA.Selected Contribution:Pulmonary hypertension in mice following intermittent hypoxia[J].J Appl Physiol,2001,90(6):2502-2507..
- 3. Koyama T,Xie Z,Gao M,et al.Adaptive changes in the capillary network in the left ventricle of rat heart[J].Jpn J Physiol,1998,48(4):229-41.
- 4. Neufeld G,Cohen T,Gengrinovitch S,et al.Vascular endothelial growth factor (VEGF) and its receptors[J].FASEB J,1999,13(1):9-22..
- 5. Liu LX,Lu H,Luo YX,et al.Stabilization of vascular endothelial growth factor mRNA by hypoxia-inducible factor 1[J].Biochem Biophys Res Commun,2002,291(4):908-914..
- 6. Banai S,Shweiki D,Pinson A,et al.Upregulation of vascular endothelial growth factor expression induced by myocardial ischaemia:implications for coronary angiogenes[J].Cardiovasc Res,1994,28(8):1176-1179.