Objective To evaluate the effect on microvessel density (MVD) and vascular endothelial growth factor (VEGF) expression of combining radiofrequency ablation (RFA) with arsenious acid (AA) locally treating liver VX2 tumor in rabbits.
Methods Twenty-eight New Zealand White rabbits with implanted liver VX2 tumors were randomly divided into four groups, control group (n=7), AA group (n=7), RFA group (n=7) and combination (RFA+AA) group (n=7). All rabbits were killed 14 days after treatment. MVD and VEGF expression were examined by immunohistochemistry.
Results The MVD degraded one by one in control group,AA group,RFA group and RAF+AA group, which were (38.50±0.44), (23.07±0.47), (18.65±0.39) and (11.36±0.36)/HP respectively, compared while each two groups, P＜0.05. The VEGF expression also degraded one by one, the ratio of positive cases were 7/7, 5/7, 4/7 and 2/7 respectively, compared while each two groups, P＜0.05. There was positive correlation between VEGF expression and MVD (Person conefficient of product-moment correlation r=0.47, P＜0.01).
Conclusion Combining RAF with AA therapy can greatly decrease MVD and VEGF expression of tumor tissue.
Citation：
ZHANG Menghui,KONG Xianbing,WANG Qiaoling,YIN Weihong,XUAN Shijin. Effect on Microvessel Density and Vascular Endothelial Growth Factor Expression of Radiofrequency Ablation with Arsenious Acid on Liver VX2 Tumors. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2007, 14(1): 19-22. doi:
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- 1. Wong SL, Edwards MJ, Chao C, et al. Radiofrequency ablation for unresectable hepatic tumors [J]. Am J Surg, 2001; 182(6)∶552.
- 2. 郭卫平， 刘燕， 王执民, 等. 兔肝VX2移植癌改良模型的建立 [J]. 中国医学影像技术， 2002； 18（5）∶397.
- 3. 陈主初， 吴端生主编. 实验动物学 [M]. 第16版. 长沙： 湖南科学技术出版社， 2003∶268～269.
- 4. Weidner N. Current pathologic methods for measuring intratumoral microvessel density within breast carcinoma and other solid tumors [J]. Breast Cancer Res Treat, 1995; 36(2)∶169.
- 5. Weidner N. Intratumor microvessel density as a prognostic factor in cancer [J]. Am J Pathol, 1995; 147(1)∶9.
- 6. Folkman J. Tumor angiogenesis [J]. Adv Cancer Res, 1985; 43∶175.
- 7. Maeda K, Chung YS, Takatsuka S, et al. Tumor angiogenesis as a predictor of recurrence in gastric carcinoma [J]. J Clin Oncol, 1995; 13(2)∶477.
- 8. Weidner N, Carroll PR, Flax J, et al. Tumor angiogenesis correlates with metastasis in invasive prostate carcinoma [J]. Am J Pathol, 1993; 143(2)∶401.
- 9. Ozpolat B, Lopez-Berestein G, Mehta K. ATRA(ouble) in the treatment of acute promyelocytic leukemia [J]. J Biol Regul Homeost Agents, 2001; 15(2)∶107.
- 10. Ferrara N, Davis-Smyth T. The biology of vascular endothelial growth factor [J]. Endocr Rev, 1997; 18(1)∶4.
- 11. Brown LF, Lanir N, McDonagh J, et al. Fibroblast migration in fibrin gel matrices [J]. Am J Pathol, 1993; 142(1)∶273.
- 12. 李江涛, 区庆嘉, 魏箐. 三氧化二砷诱导肝癌细胞凋亡的初步研究 [J]. 癌症, 2000; 19(12)∶1087.
- 13. 华海清, 秦叔逵, 王锦鸿. 三氧化二砷抗肿瘤血管形成研究 [J]. 世界华人消化杂志， 2004; 12(1)∶27.
