【Abstract】ObjectiveTo detect p27 expression in rectal carcinoma and serum transforming growth factor-β1 (TGF-β1) level in these patients, and to elucidate the modulatory effect of serum TGF-β1 on p27 expression in rectal carcinoma.
MethodsExpression of p27 was measured in 37 cases of rectal carcinoma, 22 of rectal adenoma and 19 of normal control specimens by immunohistochemical staining using antibodies to p27. Serum level of TGFβ1 was measured in these patients by enzymelinked immunosorbent assay (ELISA) method.
Resultsp27 protein was expressed in normal rectal tissue, rectal adenoma and rectal carcinoma, and the positive rate was 89.47%, 90.91% and 64.87%, respectively. The positive rate of p27 in rectal carcinoma was significantly lower than that of normal rectal tissue and rectal adenoma (P=0.025). p27 was mainly located in nucleolus of normal rectal tissue and rectal adenoma, and the positive rate of p27 in cytoplasm of rectal carcinoma was higher than that of normal and rectal adenoma. The positives rates of serum TGF-β1 in normal group, rectal adenoma group and rectal carcinoma group were 21.05%, 27.27% and 51.35%(P=0.045),respectively. The expression of p27 related to histological differentiation, lymph node metastasis and infiltration depth. Serum level of TGF-β1 related to lymph node metastasis, infiltrated depth and CEA level. The positive rate of p27 in TGF-β1 negative group and positive group was 88.89% and 42.11%(MantelHaenszel χ2=6.755,P=0.009), respectively.
ConclusionTGF-β1 may be useful in assessment of malignance and prognosis of rectal carcinoma. TGF-β1 can downregulate p27 expression in rectal carcinoma.
Citation：
JING Changqing,LI Leping,LI Zhao,WU Taihuang,MU Qingling.. Expression of p27 in Rectal Carcinoma and Modulatory Relation Between Transforming Growth Factor-β1 and p27. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(1): 33-36. doi:
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- 4. Palmqvist R, Stenling R, Oberg A, et al. Prognostic significance of p27(Kip1) expression in colorectal cancer: a clinicopathological characterization [Ｊ]. J Pathol， 1999; 188(1)∶18.
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- 6. Fan G, Ma X, Wong PY, et al. p53 dephosphorylation and p21(Cip1/Waf1) translocation correlate with caspase3 activation in TGFbeta1induced apoptosis of HuH7 cells [Ｊ]. Apoptosis， 2004; 9(2)∶211.
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