ObjectiveTo explore the regulation of nuclear factorκB (NFκB) on tumor necrosis factorα (TNFα) expression in the liver and its role in liver injury in rats with acute pancreatitis.MethodsSeventytwo Wistar rats were randomly divided into three groups: acute pancreatitis group (AP), acute pancreatitis treated with pyrrolidine dithiocarbamate (PDTC) group (APP) and sham operation group (SO). The hepatic NFκB activities were determined with electrophoretic mobility shift assays. The expressions of hepatic TNFα mRNA were detected with RTPCR. The levels of serum alanine aminotransferase (ALT) were also measured.ResultsThe NFκB activities were significantly higher in AP and APP groups than those in SO group 3-6 hours after operation. The expressions of TNFα mRNA were ber in AP and APP groups than those in SO group 3-24 hours after operation. The levels of serum ALT were also significantly higher in these two groups than those in SO group 3-24 hours after operation. However, compared with AP group, the activities of NFκB, the expressions of TNFα mRNA and the levels of ALT significantly decreased in APP group.ConclusionThe activation of hepatic NFκB is associated with the liver injury by regulating TNFα mRNA expression in acute pancreatitis.
Citation：
SHI Li,TIAN Fuzhou,LI Xu,HUANG Darong,ZHAO Bi,GU Dayong.. Regulation of Nuclear FactorKappa B on Tumor Necrosis Factorα Expression in the Liver and Its Role in Liver Injury in Rats with Acute Pancreatitis. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(3): 228-230. doi:
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- 1. Miyahara S, Isaji S. Liver injury in acute pancreatitis and mitigation by continuous arterial infusion of an antibiotic via the superior mesenteric artery [Ｊ]. Pancreas, 2001; 23(2)∶204.
- 2. 石力， 黄大熔， 田伏洲， 等. 急性胰腺炎大鼠肝脏核因子κB活化与肝损伤的关系 [Ｊ]. 世界华人消化杂志， 2002； 10（5）∶554.
- 3. Jaffray C, Yang J, Norman J. Elastase mimics pancreatitisinduced hepatic injury via inflammatory mediators [Ｊ]. J Surg Res, 2000; 90(2)∶95.
- 4. Deryckere F, Gannon F. A onehour minipreparation technique for extraction of DNAbinding proteins from animal tissues [Ｊ]. Biotechniques, 1994; 16(3)∶405.
- 5. Chomczynski P, Sacchi N. Singlestep method of RNA isolation by acid guanidinium thiocyanatephenolchloroform extraction [Ｊ]. Anal Biochem, 1987; 162(1)∶156.
- 6. Pitchumoni CS, Agarwal N, Jain NK. Systemic complications of acute pancreatitis [Ｊ]. Am J Gastroenterol, 1988; 83(6)∶597.
- 7. Valen G, Yan ZQ, Hansson GK. Nuclear factor kappaB and the heart [Ｊ]. J Am Coll Cardiol, 2001; 38(2)∶307.
- 8. Wright G, Singh IS, Hasday JD, et al. Endotoxin stressresponse in cardiomyocytes: NFkappa B activation and tumor necrosis factoralpha expression [Ｊ]. Am J Physiol Heart Circ Physiol, 2002; 282(3)∶H872.
- 9. Moine P, McIntyre R, Schwartz MD, et al. NFkappa B regulatory mechanisms in alveolar macrophages from patients with acute respiratory distress syndrome [Ｊ]. Shock, 2000; 13(2)∶85.
- 10. Udalova IA, Knight JC, Vidal V, et al. Complex NFkappa B interactions at the distal tumor necrosis factor promoter region in human monocytes [Ｊ]. J Biol Chem, 1998; 273(33)∶21178.
- 11. Essani NA, Fisher MA, Jaeschke H. Inhibition of NFkappa B activation by dimethyl sulfoxide correlates with suppression of TNFalpha formation, reduced ICAM1 gene transcription, and protection against endotoxininduced liver injury [Ｊ]. Shock, 1997; 7(2)∶90.
