ObjectiveTo establish a mouse model of acute necrotizing pancreatitis.MethodsThirty-six male ICR mice were randomly divided into control group (n=6) and experimental group (n=30). Each of the animals in the experimental group received 7 intraperitoneal injections of caerulein (50 μg/kg body weight) in 0.9% NaCl at hourly intervals over 6 hours. The animals in the experimental group were killed at 9,18,24,48 and 72 hours respectively after the first caerulein injection. The control animals received the same volume of 0.9% NaCl without caerulein. The animals in the control group were killed at the 18th hour after the first intraperitoneal injection. The severity of acute necrotizing pancreatitis was evaluated in terms of amylase level, pancreatic weight/body weight and the histological changes. Variance analysis was employed in the processing of these data. ResultsBoth amylase level and pancreatic weight elevated 9 hours after the first caerulein injection, and correlated with the course of pancreatitis. The maximums of both alterations were observed at the same time point (18 hours after the first injection of caerulein). Prominent interstitial inflammation and acinar cell necrosis occurred at the 18th hour, and the histological score for pancreatitis reached a maximum （P＜0.05）. Conclusion Intraperitoneal injection of a large dosage of caerulein can induce acute necrotizing pancreatitis in ICR mice. This method is simple and noninvasive, and the model established thus is stable and reproducible.
Citation：
LI Quansheng,CHEN Xiaoli,ZHOU Zongguang,LIU Xubao,ZHANG Zhaoda.. Experimental Study on the Mouse Model of Acute Necrotizing Pancreatitis Induced by Intraperitoneal Injection of Caerulein. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2004, 11(4): 335-337. doi:
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赵慧业，肖国衡，熊启达，等. 胰实质内注射牛黄胆酸钠引起实验性大鼠急性出血性胰腺炎模型及其早期损害的研究 [Ｊ]. 中国病理生理杂志， 1986； 2（2）∶112.
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Tani S, Itoh H, Okabayashi Y, et al. New model of acute necrotizing pancreatitis induced by excessive doses of arginine in rats[Ｊ]. Dig Dis Sci, 1990; 35(3)∶367.
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Vasilescu C, Herlea V, Buttenschoen K, et al. Endotoxin translocation in two models of experimental acute pancreatitis [Ｊ]. J Cell Mol Med, 2003; 7(4)∶417.
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Watanabe O, Baccino FM, Steer ML, et al. Supramaximal caerulein stimulation and ultrastructure of rat pancreatic acinar cell: early morphological changes during development of experimental pancreatitis [Ｊ]. Am J Physiol, 1984; 246(4 Pt 1)∶G457.
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| 10. |
Tani S, Otsuki M, Itoh H,et al. Histologic and biochemical alterations in experimental acute pancreatitis induced by supramaximal caerulein stimulation [Ｊ]. Int J Pancreatol, 1987; 2(5-6)∶337.
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- 1. Niederau C, Ferrell LD, Grendell JH. Caeruleininduced acute necrotizing pancreatitis in mice: protective effects of proglumide, benzotript, and secretin [Ｊ]. Gastroenterology， 1985; 88(5 Pt 1)∶1192.
- 2. van Laethem JL, Eskinazi R, Louis H, et al. Multisystemic production of interleukin 10 limits the severity of acute pancreatitis in mice [Ｊ]. Gut, 1998; 43(3)∶408.
- 3. 刘兴，陈怀仁，杨得同，等. 蛙皮素致小鼠急性坏死性胰腺炎模型 [Ｊ]. 南京铁道医学院学报，1996； 15（4）∶286.
- 4. 薛建国，王宇，许元弟. 建立大鼠急性出血坏死性胰腺炎模型方法的改进 [Ｊ]. 中华实验外科杂志， 1994； 11（5）∶313.
- 5. 赵慧业，肖国衡，熊启达，等. 胰实质内注射牛黄胆酸钠引起实验性大鼠急性出血性胰腺炎模型及其早期损害的研究 [Ｊ]. 中国病理生理杂志， 1986； 2（2）∶112.
- 6. Lombardi B, Estes LW, Longnecker DS. Acute hemorrhagic pancreatitis (massive necrosis) with fat necrosis induced in mice by DLethionine fed with a cholinedeficient diet [Ｊ]. Am J Pathol, 1975; 79(3)∶465.
- 7. Tani S, Itoh H, Okabayashi Y, et al. New model of acute necrotizing pancreatitis induced by excessive doses of arginine in rats[Ｊ]. Dig Dis Sci, 1990; 35(3)∶367.
- 8. Vasilescu C, Herlea V, Buttenschoen K, et al. Endotoxin translocation in two models of experimental acute pancreatitis [Ｊ]. J Cell Mol Med, 2003; 7(4)∶417.
- 9. Watanabe O, Baccino FM, Steer ML, et al. Supramaximal caerulein stimulation and ultrastructure of rat pancreatic acinar cell: early morphological changes during development of experimental pancreatitis [Ｊ]. Am J Physiol, 1984; 246(4 Pt 1)∶G457.
- 10. Tani S, Otsuki M, Itoh H,et al. Histologic and biochemical alterations in experimental acute pancreatitis induced by supramaximal caerulein stimulation [Ｊ]. Int J Pancreatol, 1987; 2(5-6)∶337.
