Objective To study the changes of endothelin (ET) and nitric oxide (NO) in the local site of vein transfer with delayed breaking pedicle and the relation with vasospasm and vein transfer in rabbits. MethodsThe ET concentration of blood was determined with the radioimmunoassay method. The plasma NO-2,NO-3 levels in the local site of vein transfer with delayed breaking pedicle, which reflected NO levels indirectly, were detected with Ultravioletvisible (UvVIS ) spectrophotometer. ResultsThe endothelin concentration of blood was increased significantly at 2, 4 hour after the operation (P＜0.01), and at 8 hour after the operation (P＜0.05). The plasma NO level was significantly decreased at 2, 4 hour after the operation (P＜0.01). But at 24 hour after the operation, the plasma NO level was increased significantly (P＜0.05). Conclusion The recovery of ET concentration of blood and the increase of plasma NO at 24 hour after the operation are the cause of the reduced incidence of vascular crisis of vein transfer with delayed breaking pedicle, and the very time point is the optimum moment for pedicle breaking.
Citation：
WANG Lihua,ZHEN Shusen,YU Yaosheng,et al.. Effect of Endothelin and Nitric Oxide Changes in Local Site of Vein Transfer with Delayed Breaking Pedicle in Rabbits. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2002, 9(4): 235-237. doi:
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- 1. Hirata Y, Itoh K, Ando K, et al. Plasma endothelin levels during surgery [J]. N Engl J Med, 1989; 321(24)∶1686.
- 2. 段志泉, 张强著. 实用血管外科 [M]. 第 1版. 沈阳： 辽宁科学技术出版社，1999∶1～55.
- 3. Stoyanovsky D, Murphy T, Anno PR, et al. Nitric oxide activates skeletal and cardiac ryanodine receptors [J]. Cell Calcium, 1997; 21(1)∶19.
- 4. Wagner OF, Christ G, Wojta J, et al. Polar secretion of endothelin1 by cultured endothelial cells [J]. J Biol Chem, 1992; 267(23)∶16066.
- 5. Kourembanas S, Marsden PA, McQuillan LP, et al. Hypoxia induces endothelin gene expression and secretion in culture human endothelium [J]. J Clin Invest, 1991; 88(3)∶1054.
- 6. Morganti A, Giussani M, Ghio F, et al. Endothelinreleasing stimuli and calcium antagonists in normal and pathological conditions [J]. J Hypertention Suppl, 1994; 12 (1)∶S27.
- 7. Poderoso JJ, Peralta JG, Lisdero CL, et al. Nitric oxide regulates oxygen uptake and hydrogen peroxide release by the isolated beating rat heart [J]. Am J Physiol, 1998; 274(1 Pt 1)∶C112.
- 8. Li Z, Chapleau MW, Bates JN, et al. Nitric oxide as an autocrine regulator of sodium currents in baroreceptor neurons [J]. Neuron, 1998; 20(5)∶1039.
