【Abstract】Objective To observe the changeable expressions of vascular endothelial growth factor (VEGF) and integrin β3 during the angiogenetic process of granulation tissue. Methods mRNA and protein of VEGF and integrin β3 in human normal subcutaneous tissue, proliferative granulation tissue and mature granulation tissue were observed by RT-PCR and immunohistochemistry staining. Results The expressions VEGF and integrin β3 were low in normal subcutaneous tissue and were much higher in proliferative granulation tissue. When the granulation tissue was mature, the expression was decreased again. Conclusion VEGF and integrin β3 are important regulating factors in ngiogenesis.
Citation：
ZHANG Juliang,WANG Ling,JIN Yuanchao,et al.. EXPRESSION OF VASCULAR ENDOTHELIAL GROWTH FACTOR AND INTEGRIN β3 DURING THE ANGIOGENETIC PROCESS OF GRANULATION TISSUE. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2001, 8(6): 378-380. doi:
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- 1. Folkman J. Angiogenesis in cancer, vascular, rheumatoid and other disease 〔Ｊ〕. Nature Med, 1995; 1(1)∶27.
- 2. Tischer E, Mitchell R, Hartman T, et al. The human gene for vascular endothelial growth factor. Multiple protein forms are encoded through alternative exon splicing 〔Ｊ〕. J Biol Chem, 1991; 266(18)∶11947.
- 3. Fitzgerald LA, Sleiner B, Rall SC, et al. Protein sequence of endothelial glycoprotein derived from a cDNA clone: identify with platelet glycoprotein and similariti to “integrin” 〔Ｊ〕. J Biol Chem, 1987; 262(9)∶3936.
- 4. Chomczynski P, Sacchi N. Singlestep method of RNA isolation by acid guanidinium thiocyanatephenolchloroform extraction 〔Ｊ〕. Anal Biochem, 1987; 162(1)∶ 156.
- 5. Dvorak HF, Brown LF, Detmar M, et al. Vascular permeability factor/vascular endothelial growth factor, microvascular hyperpermeability, and angiogenesis 〔Ｊ〕. Am J Pathol, 1995; 146(5)∶1029.
- 6. Horak ER, Leek R, Klenk N, et al. Angiogenesis, assessed by platelet/endothelial cell adhesion molecule antibodies, as indicator of node metastasis and survival in breast cancer 〔Ｊ〕. Lancet, 1992; 340(8828)∶1120.
- 7. Brooks PC, Clark RA, Cheresh DA. Requirement of vascular integrin alpha v beta 3 for angiogenesis 〔Ｊ〕. Science, 1994; 264(5158)∶569.
- 8. Goede V, Schmidt T, Kimmina S, et al. Analysis of blood vessel maturation processes during cyclic ovarian angiogenesis 〔Ｊ〕. Lab Invest, 1998; 78(11)∶1385.
- 9. Isner JM, Baumgartner I, Rauh G, et al. Treatment of thromboangiitis obliterans (Buergers disease) by intramuscular gene transfer of vascular endothelial growth factor: preliminary clinical results 〔Ｊ〕. J Vasc Surg, 1998; 28(6)∶964.
