Objective To establish a reliable rats model of orthotopic liver transplantation with non-heart beating donors. Methods The model was established with modified double-cuff method. According to obtain pre-liver warm ischemia time experiencing non-heart-beat the rats were divided into 3 groups: 10 min (R10 group), 20 min (R20 group) and 30 min (R30 group), then one week survival after operation was compared in rats.
Results The operative time of donor was 30 min approximately except warm ischemia time and the cold preservation time of donor liver was 1 h. The anastomotic time for suprahepatic vena cava was 12-22 min (mean 15 min). The anastomotic time for portal vein and infrahepatic vena cava was about 2 min and 1 min, respectively. The anhepatic phase sustained 14-24 min (mean 19 min). The operative time of receptor was 50-65 min (mean 60 min). Twelve rats died at 24 h after operation, which was considered as operative failure. The success rates of operation in R10 group, R20 group, and R30 group were 95% (19/20), 80% (16/20), and 65% (13/20), respectively. After one week the survival rate was 95% (18/19), 81% (13/16), and 54% (7/13), respectively. Conclusions Improved non-heart donor liver transplantation model of rat on the basis of Kamada’s “twocuff technique” acts as a good simulation in clinical non-heart-donor liver transplantation. This study showes that rat liver can tolerate warm ischemia time less than 30 min, the short-term survival after transplantation can reach satisfactory results. However, long-term survival requires further study.
Citation：
LI Jingfeng,DING Huanxin,BAI Jianhua,LI Xiaoyan,GUAN Aoran.. Surgical Skills of Non-Heart-Beat Liver in Rat Liver Transplantation Model. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2010, 17(8): 785-788. doi:
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Copyright © the editorial department of CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY of West China Medical Publisher. All rights reserved
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- 1. 陈志红, 钱海鑫, 秦磊, 等. 改良二套管法大鼠原位肝移植的围术期管理 [J]. 江苏大学学报(医学版), 2009; 19(4): 360361, 365.
- 2. 张升宁, 李立, 冉江华, 等. 原位肝移植术中动脉重建及术后并发症的防治 [J]. 中国普外基础与临床杂志, 2007; 14(5): 564568.
- 3. 刘骅, 曹晖, 吴志勇. 改良二袖套法大鼠原位肝移植180例 [J]. 消化外科, 2006; 5(1): 5256.
- 4. 熊力, 王玉柱, 叶启发, 等. 大鼠自体原位肝移植灌注模型的技术改良 [J]. 中国普通外科杂志, 2008; 17(7): 716717.
- 5. 侍阳, 周晓俊, 殷骏, 等. 二袖套法大鼠原位肝移植50例 [J]. 徐州医学院学报, 2007; 27(9): 588591.
- 6. Kamada N, Calne RY. A surgical experience with five hundred thirty liver transplants in the rat [J]. Surgery, 1983; 93(1 Pt 1): 6469.
- 7. 彭勇, 龚建平, 刘长安, 等. 大鼠原位肝移植模型制作过程中麻醉方法的选择 [J]. 中国普通外科杂志, 2003; 12(9): 673676.
- 8. 宇汝胜, 汪泳, 钱海鑫. 大鼠原位肝移植模型的手术操作技巧探讨 [J]. 肝胆胰外科杂志, 2008; 20(1): 79.
- 9. 李军, 窦科峰, 赵青川, 等. 两套袖法大鼠原位肝移植模型的建立及改进 [J]. 第四军医大学学报, 2006; 27(9): 835837.
- 10. 马毅, 何晓顺, 陈规划, 等. 不同热缺血时间对大鼠移植肝功能状态及存活期的影响 [J]. 中华实验外科杂志, 2003; 20(4): 322324, I002.
- 11. 张宝良, 袁庆鑫, 刘彤. 大鼠心脏停搏供体动脉化原位肝移植模型制作体会 [J]. 肝胆胰外科杂志, 2009; 21(3): 177179.
- 12. Monbaliu D, Libbrecht L, De Vos R, et al. The extent of vacuolation in nonheartbeating porcine donor liver grafts prior to transplantation predicts their viability [J]. Liver Transpl, 2008; 14(9): 12561265.
