【Abstract】Objective To explore the effect against gastric cancer induced by Newcastle disease virus modified autologous tumor vaccine （NDV-ATV）pulsed dendritic cells(DCs).
Methods The Newcastle disease virus infected the gastric cancer lines (MNK45) and was lost its activity. Peripheral blood mononuclear cell (PBMC) were cultured under condition of recombinant human granulocyte macrophage-colony stimulating factor (1 000 u/ml)+IL-4(1 000 u/ml) + TNF-α(100 ng/ml). The tumor antigen specific cytotoxic T lymphocytes (CTL) was generated from activated autologous T cell by the Newcastle disease virus infected the MNK45 pulsed DC. And Cyto Tox 96TM in vitro assayed the cytotoxicity of CTL to MNK45. Thawed gastric cancer cell antigen were used as control in these experiments.
Results The killing rate of MNK45 by antigen specific CTL reached (90.15±9.82)%, which was nearly twice as high as that of control(60.57±5.74)%. The CTL had much higher cytotoxicity to different differentiated type of gastric cancer cells such as MGC803〔(52.23±6.45)% 〕 and SGC7901〔 (61.75±8.84)%〕, as compared with LOVO〔(9.11±3.42)%〕 and HepG2 〔 (8.30±3.12)%〕tumor cells(P＜0.05).
Conclusion Efficient and specific of against gastric cancer immunoreaction can be induced in virtue of NDV-ATV pulsed DCs, NDV-ATV loaded DCs might provide a new kind of theraputic means for gastric cancer.
Citation：
WU Miao,YU Peiwu,ZENG Dongzhu,LEI Xiao,ZHAO Yongliang,ZHU Ziman,WANG Ziqiang.. Study on Anti-Gastric Cancer Effects Induced by NDV-ATV and Dendritic Cells. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2005, 12(4): 380-383. doi:
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- 1. Termeer CC, Schirrmacher V, Brocker EB, et al. Newcastle disease virus infection induces B71/B72independent Tcell costimulatory activity in human melanoma cells [Ｊ]. Cancer Gene Ther, 2000; 7(2)∶316.
- 2. Nair SK, Snyder D, Rouse BT, et al. Regression of tumors in mice vaccinated with professional antigenpresenting cells pulsed with tumor extracts [Ｊ]. Int J Cancer, 1997; 70(6)∶706.
- 3. 吴淼，余佩武，曾冬竹，等. 胃癌患者术后NDVATV治疗对免疫功能的影响 [Ｊ]. 实用癌症杂志， 2003; 18(2)∶179.
- 4. 吴淼，余佩武，蔡志民，等. 负载酸洗抗原树突状细胞诱导高效特异的抗胃癌细胞效应 [Ｊ]. 第三军医大学学报， 2002; 24(10)∶1236.
- 5. 雷晓，余佩武，石彦，等. 树突状细胞肿瘤疫苗诱导抗胃癌作用的实验研究 [Ｊ]. 中华胃肠外科杂志，2002; 5(1)∶45.
- 6. Dauer M, Obermaier B, Herten J, et al. Mature dendritic cells derived from human monocytes within 48 hours: a novel strategy for dendritic cell differentiation from blood precursors [Ｊ]. J Immunol, 2003; 170(8)∶4069.
- 7. 巴德年主编. 当代免疫学技术与应用 [M]. 第1版. 北京： 北京医科大学中国协和医科大学联合出版社， 1998∶158~167.
- 8. Ladhams A, Schmidt C, Sing G, et al. Treatment of nonresectable hepatocellular carcinoma with autologous tumorpulsed dendritic cells [Ｊ]. J Gastroenterol Hepatol, 2002; 17(8)∶889.
- 9. Bodey B, Siegel SE, Kaiser HE. Antigen presentation by dendritic cells and their significance in antineoplastic immunotherapy [Ｊ]. In Vivo, 2004; 18(1)∶81.
- 10. Yang L, Carbone DP. Tumorhost immune interactions and dendritic cell dysfunction [Ｊ]. Adv Cancer Res, 2004; 92∶13.
- 11. Maehara Y, Tomisaki S, Oda S, et al. Lymph node metastasis and relation to tumor growth potential and local immune response in advanced gastric cancer [Ｊ]. Int J Cancer, 1997; 74(2)∶224.
