Objective To find the relation between donor liver cold preservation-reperfusion injury and hepatocellular apoptosis during liver transplantation.
Methods Four groups of rabbit livers, which had experienced cold storage for 0,3,6,9hr respectively, were observed during their cold preservation-reperfusion by using TdT-mediated dUTP-biotion nickend labeling and electron microscope.
Results Apoptopic hepatic parenchymal cells were obviously observed in reperfusing livers subsequent to cold storage. Furthermore, the longer the cold storage duration, the greater the number of apoptotic cells. On the contrary, no or rare apoptotic hepatic parenchymal cells was observed in all the groups at the end of cold preservation.
Conclusion It suggests that apoptosis of hepatic parenchymal cells is markedly involved in donor liver cold preservation-reperfusion injury.
Citation:
TANG Lijun,TIAN Fuzhou,WANG Y,et al.. OBSERVATION ON HEPATOCELLULAR APOPTOSIS DURING COLD PRESERVATION-REPERFUSION OF ISOLATED LIVERS IN RABBITS. CHINESE JOURNAL OF BASES AND CLINICS IN GENERAL SURGERY, 2000, 7(4): 214-216. doi:
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- 1. Jamieson NV, Lindell S, Sundberg R, et al. An analysis of the components in UW solution using the isolated perfused rabbit liver 〔J〕. Transplantation, 1988; 46(4)∶512.
- 2. 汤礼军, 田伏洲, 王雨等. 兔肝脏保存再灌注损伤模型的建立及评价 〔J〕. 肝胆外科杂志, 1998; 6(2)∶123.
- 3. Gavrieli Y, Sherman Y, BENSasson SA. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation 〔J〕. J Cell Biol, 1992; 119(1)∶493.
- 4. Shah KA, Shurey S, Green CJ. Characterization of apoptosis in intestinal ischemia reperfusion injurya light and eletron microscopic study 〔J〕. Int J Exp Pathol, 1997; 78(5)∶355.
- 5. GraslKraupp B, RuttkayNedecky B, Koudelka H, et al. In situ detection of fragmented DNA (Tunel assay) fails to discriminate among apoptosis, necrosis and autolytic cell death: a cautionary note 〔J〕. Hepatology, 1995; 21(5)∶1465.
- 6. Schumer M, Colombel MC, Sawczuk IS, et al. Morphologic biochemical and molecular evidence of apoptosis during the reperfusion phase after brief periods renal ischemia 〔J〕. Am J Pathol, 1991; 140(4)∶831.
- 7. Gantner F, Leist M, Jilg S, et al. Tumor necrosis factorinduced hepatic DNA fragmentation as an early marker of T celldependent liver injury in mice 〔J〕. Gastroenterology, 1995; 109(1)∶166.
- 8. 印彤, 童善庆, 谢玉才等. 超抗原SEB和D氨基半乳糖对小鼠肝细胞的作用观察 〔J〕. 免疫学杂志, 1998; 14(1)∶36.