Objective To observe barrier function changes of gut mucosa in rats with acute respiratory distress syndrome(ARDS)．Methods Forty SD rats were randomized to an experiment group (n：30)and a control group(n=10)．Oleic acid was injected via vena femoralis to establish ARDS ratmode1．Subgroups in the experiment group were randomly divided by time 30 min，2 h，4 h interval after injection(n=10 in each subgroup)．Concentration of D-lactate and endotoxin and activity of diamine
oxidase in blood plasma were measured．Histopathological changes of small intestine were observed under light microscope．Results Compared with the control group，the activation of diamine oxidase in the experiment group was higher after 30 min of injection(P lt;0.01)．Concentration of D-lactate，the activity of
diamine oxidase and endotoxin level in the experiment group were all elevated after 2 hours of injection(all P lt;0.05)，and further elevated after 4 hours．In the rats’villous interstitial after 2 hours of the injection，there were edema，hyperemia，and infiltration of neutrophils，eosinophils and lymphocytes．After 4 hours of
the injection，the villous epithelium showed desquamation，necrosis，denaturalization and erosion，associated with infihration of lymphocytes and neutrophils in the mucosa．Conclusion In oleic acid-induced ARDS．permeability of gut mueosa increases and gut barrier is dysfunctional．
Citation：
CHEN Yingchun,LUO Yong,HAN Feng,YANG Tianyun. An experimental study on the gut mucosal barrier in rats with acute respiratory distress syndrome. Chinese Journal of Respiratory and Critical Care Medicine, 2008, 08(6): 467-469. doi:
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Copyright © the editorial department of Chinese Journal of Respiratory and Critical Care Medicine of West China Medical Publisher. All rights reserved
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Nuckton TJ，Alonso JA，Kalllet RH，et al．Pulmonary dead．space fraction as a risk factor for death in the acute respiratory distress syndrome．N Engl J Med.2002．346：1281．.
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Zilberberg MD，Epstein SK．Acute lung injury in the medical ICU：comorbid condictions，age，etiology，and hospital outcome．Am J Respir Crit Care Med，1998，157：1159．.
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陈成，黄建安．急性肺损伤／急性呼吸窘迫综合征预后评估指标研究进展．中国呼吸与危重监护杂志，2008，7：156-160．.
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宋鹏，李琦，王长征，等．17例重症急性呼吸窘迫综合征临床资料回顾性分析．中国呼吸与危重监护杂志。2006．5：35-38．.
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Burton F，Dickey MD，Roger S，et a1．Oleic-acid-induced lung injury in the rat．Am J Pathol，1981，376-383．.
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Grotjohan HP，van der Heijde RM，Jansen JR。et a1．A stable model of respiratory distress syndrome by small injections of oleic acid in pigs．Intensive Care Med，1996，22：336-344．.
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De Witt RC，Kudsk KA．The gut’s role in metabolism．mucosal barrier function，and gut immunology．Infect Dis Clin North Am，1999，13：465-481．.
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Schmidt H．Martindale R．The gastrointestinal tract in critical illness．Curt Opin Clin Nutr Metab Care，2001，4：547-551．.
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Fink MP．Intestinal epithelial hyperpermeability：update on the pathogenesis of gut mucosal barrier dysfunction in critical illness．Corr Opin Crit Care，2003，9：143-151．.
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| 10. |
Ammori BJ．Role of the gut in the course of severe acute pancreatitis．Pancreas，2003，26：122-129．.
|
| 11. |
Monti P，Wysocki J，vall der Meeren A，et al．The contribution of radiation-induced injury to the gastrointestinal tract in the development of multi-organ dysfunction syndrome or failure．BJR，2005，27：89-94．.
|
| 12. |
Sun Z，Wang X，Wallen R，et al．The influence of apoptosis on intesti．hal barrier integrity in rats．Scand J Gastroenterol，1998，33：415-422．.
|
| 13. |
黎君友，吕艺，晋桦，等．创伤后血二胺氧化酶的变化与肠黏膜损伤．氨基酸和生物资源，1999，21：17-19．.
|
| 14. |
Salzman AL，Wang H，Wollert PS，et al．Endotoxin．induced ileal mucosal hyperpermeability in pigs：role of tissue acidosis．Am J Physiol．1994，266(4 Pt 1)：G633．G646．.
|
| 15. |
Hemandez LA，Grisham MB，Twohig JB，et a1．Role of nentrophils in ischem iareperfusion indued microvasadar injury．Am J Physiol．1987，253：699．.
|
| 16. |
玛依努尔·伊明尔艾山，张建龙，雪合热提·伊纳也提．油酸致ARDS过程中小,肠脂质过氧化和一氧化氮的动态变化．新疆医科大学学报，2003，26：23．.
|
| 17. |
Grotz MRW ，Deitch EA，Ding J，et a1．Intestinal cytokine response after gut ischemia：role of gut barrier failure．Ann Surg，1999，299：478-486．.
|
| 18. |
Gong JP，Wu CX，Liu CA，et al．Intestinal damage mediated by kupfer cells in rats with endotoxemia．World J Gastmenterol，2002，8：923-927．.
|
| 19. |
Deitch EA．The role of intestinal barrier failure and bacterial translocation in the development of systemic infection and multiple organ failure．Arch Surg，1990， 125：403-404．.
|
| 20. |
Magnotti LJ，Deitch EA．Bums，bacterial translocation，gut barrier function，and failure．J Bum Care Rehabil，2005，26：383-391．.
|
- 1. Nuckton TJ，Alonso JA，Kalllet RH，et al．Pulmonary dead．space fraction as a risk factor for death in the acute respiratory distress syndrome．N Engl J Med.2002．346：1281．.
- 2. Zilberberg MD，Epstein SK．Acute lung injury in the medical ICU：comorbid condictions，age，etiology，and hospital outcome．Am J Respir Crit Care Med，1998，157：1159．.
- 3. 陈成，黄建安．急性肺损伤／急性呼吸窘迫综合征预后评估指标研究进展．中国呼吸与危重监护杂志，2008，7：156-160．.
- 4. 宋鹏，李琦，王长征，等．17例重症急性呼吸窘迫综合征临床资料回顾性分析．中国呼吸与危重监护杂志。2006．5：35-38．.
- 5. Burton F，Dickey MD，Roger S，et a1．Oleic-acid-induced lung injury in the rat．Am J Pathol，1981，376-383．.
- 6. Grotjohan HP，van der Heijde RM，Jansen JR。et a1．A stable model of respiratory distress syndrome by small injections of oleic acid in pigs．Intensive Care Med，1996，22：336-344．.
- 7. De Witt RC，Kudsk KA．The gut’s role in metabolism．mucosal barrier function，and gut immunology．Infect Dis Clin North Am，1999，13：465-481．.
- 8. Schmidt H．Martindale R．The gastrointestinal tract in critical illness．Curt Opin Clin Nutr Metab Care，2001，4：547-551．.
- 9. Fink MP．Intestinal epithelial hyperpermeability：update on the pathogenesis of gut mucosal barrier dysfunction in critical illness．Corr Opin Crit Care，2003，9：143-151．.
- 10. Ammori BJ．Role of the gut in the course of severe acute pancreatitis．Pancreas，2003，26：122-129．.
- 11. Monti P，Wysocki J，vall der Meeren A，et al．The contribution of radiation-induced injury to the gastrointestinal tract in the development of multi-organ dysfunction syndrome or failure．BJR，2005，27：89-94．.
- 12. Sun Z，Wang X，Wallen R，et al．The influence of apoptosis on intesti．hal barrier integrity in rats．Scand J Gastroenterol，1998，33：415-422．.
- 13. 黎君友，吕艺，晋桦，等．创伤后血二胺氧化酶的变化与肠黏膜损伤．氨基酸和生物资源，1999，21：17-19．.
- 14. Salzman AL，Wang H，Wollert PS，et al．Endotoxin．induced ileal mucosal hyperpermeability in pigs：role of tissue acidosis．Am J Physiol．1994，266(4 Pt 1)：G633．G646．.
- 15. Hemandez LA，Grisham MB，Twohig JB，et a1．Role of nentrophils in ischem iareperfusion indued microvasadar injury．Am J Physiol．1987，253：699．.
- 16. 玛依努尔·伊明尔艾山，张建龙，雪合热提·伊纳也提．油酸致ARDS过程中小,肠脂质过氧化和一氧化氮的动态变化．新疆医科大学学报，2003，26：23．.
- 17. Grotz MRW ，Deitch EA，Ding J，et a1．Intestinal cytokine response after gut ischemia：role of gut barrier failure．Ann Surg，1999，299：478-486．.
- 18. Gong JP，Wu CX，Liu CA，et al．Intestinal damage mediated by kupfer cells in rats with endotoxemia．World J Gastmenterol，2002，8：923-927．.
- 19. Deitch EA．The role of intestinal barrier failure and bacterial translocation in the development of systemic infection and multiple organ failure．Arch Surg，1990， 125：403-404．.
- 20. Magnotti LJ，Deitch EA．Bums，bacterial translocation，gut barrier function，and failure．J Bum Care Rehabil，2005，26：383-391．.
